| 2021-08-07 15:00:13August 7, 2021 |   Holker Street vs Yellowfield Stags | 1 - 1 | | Recap |
| 2021-08-07 15:00:18August 7, 2021 |   Burnden Wanderers vs Amber Exiles | 0 - 1 | | Recap |
| 2021-08-07 15:00:26August 7, 2021 |   Brunton Wyverns vs Greenfield Central | 0 - 2 | | Recap |
| 2021-08-07 15:00:31August 7, 2021 |   MonoTown FC vs Blocks County | 3 - 0 | MonoTown City StadiumMonoTown | Recap |
| 2021-08-07 15:00:39August 7, 2021 |   Blundell Ballers vs Whaddon Robins | 1 - 0 | | Recap |
| 2021-08-07 16:00:25August 7, 2021 |   FC Colburn vs Prudhoe United | 0 - 2 | | Recap |
| 2021-08-07 16:00:35August 7, 2021 |   Bay City Shrimps vs Alexandra Cruisers | 2 - 0 | | Recap |
| 2021-08-07 16:00:42August 7, 2021 |   Somerton Academical vs Southsea United | 1 - 0 | | Recap |
| 2021-08-07 17:00:32August 7, 2021 |   Halifax City vs Cambricks United | 0 - 1 | | Recap |
| 2021-08-07 20:00:41August 7, 2021 |   Alton Athletic vs Birkenhead Rovers | 0 - 2 | | Recap |
| 2021-08-07 20:00:43August 7, 2021 |   Saddle Town Swifts vs Burslem Builders | 1 - 1 | | Recap |
| 2021-08-07 20:00:53August 7, 2021 |   Phoenix Shakers vs Broadfield Borough | 2 - 2 | | Recap |
| 2021-08-14 15:00:21August 14, 2021 | Burslem Builders vs MonoTown FC | 1 - 3 | | Recap |
| 2021-08-14 15:00:46August 14, 2021 | Somerton Academical vs Greenfield Central | 4 - 0 | | Recap |
| 2021-08-14 15:00:58August 14, 2021 | Cambricks United vs Brunton Wyverns | 1 - 2 | | Recap |
| 2021-08-14 16:00:43August 14, 2021 | Birkenhead Rovers vs Blundell Ballers | 1 - 1 | | Recap |
| 2021-08-14 18:00:05August 14, 2021 | Whaddon Robins vs Halifax City | 3 - 1 | | Recap |
| 2021-08-14 18:00:34August 14, 2021 | Broadfield Borough vs Alton Athletic | 0 - 2 | | Recap |
| 2021-08-14 18:00:37August 14, 2021 | Blocks County vs Phoenix Shakers | 0 - 0 | | Recap |
| 2021-08-14 19:00:14August 14, 2021 | Southsea United vs Holker Street | 1 - 0 | | Recap |
| 2021-08-14 19:00:30August 14, 2021 | Yellowfield Stags vs Burnden Wanderers | 1 - 1 | | Recap |
| 2021-08-14 19:00:33August 14, 2021 | Amber Exiles vs FC Colburn | 3 - 0 | | Recap |
| 2021-08-15 15:00:28August 15, 2021 | Prudhoe United vs Bay City Shrimps | 0 - 1 | | Recap |
| 2021-08-15 15:00:33August 15, 2021 | Alexandra Cruisers vs Saddle Town Swifts | 3 - 0 | | Recap |
| 2021-08-17 19:00:44August 17, 2021 | MonoTown FC vs Amber Exiles | 3 - 0 | MonoTown City StadiumMonoTown | Recap |
| 2021-08-17 19:30:21August 17, 2021 | Somerton Academical vs Burnden Wanderers | 0 - 3 | | Recap |
| 2021-08-17 19:30:24August 17, 2021 | Alton Athletic vs Alexandra Cruisers | 1 - 2 | | Recap |
| 2021-08-17 19:30:40August 17, 2021 | Phoenix Shakers vs Prudhoe United | 1 - 1 | | Recap |
| 2021-08-17 19:30:58August 17, 2021 | FC Colburn vs Holker Street | 1 - 1 | | Recap |
| 2021-08-17 21:00:08August 17, 2021 | Blundell Ballers vs Burslem Builders | 1 - 0 | | Recap |
| 2021-08-17 21:00:43August 17, 2021 | Saddle Town Swifts vs Yellowfield Stags | 1 - 0 | | Recap |
| 2021-08-17 21:00:49August 17, 2021 | Bay City Shrimps vs Southsea United | 3 - 0 | | Recap |
| 2021-08-17 23:00:04August 17, 2021 | Greenfield Central vs Birkenhead Rovers | 0 - 1 | | Recap |
| 2021-08-17 23:00:10August 17, 2021 | Brunton Wyverns vs Broadfield Borough | 1 - 2 | | Recap |
| 2021-08-17 23:00:21August 17, 2021 | Halifax City vs Blocks County | 0 - 1 | | Recap |
| 2021-08-17 23:00:52August 17, 2021 | Cambricks United vs Whaddon Robins | 0 - 0 | | Recap |
| 2021-08-21 15:00:04August 21, 2021 | Somerton Academical vs Yellowfield Stags | 1 - 0 | | Recap |
| 2021-08-21 15:00:18August 21, 2021 | Burnden Wanderers vs Alexandra Cruisers | 0 - 3 | | Recap |
| 2021-08-21 15:00:23August 21, 2021 | MonoTown FC vs Birkenhead Rovers | 2 - 1 | MonoTown City StadiumMonoTown | Recap |
| 2021-08-21 15:00:27August 21, 2021 | Bay City Shrimps vs Blocks County | 1 - 2 | | Recap |
| 2021-08-21 15:00:30August 21, 2021 | Southsea United vs Amber Exiles | 1 - 2 | | Recap |
| 2021-08-21 15:00:33August 21, 2021 | Saddle Town Swifts vs Broadfield Borough | 2 - 1 | | Recap |
| 2021-08-21 15:00:43August 21, 2021 | Holker Street vs Prudhoe United | 3 - 1 | | Recap |
| 2021-08-21 17:00:01August 21, 2021 | Halifax City vs Brunton Wyverns | 17:00:015:00 pm | | Recap |
| 2021-08-21 17:00:17August 21, 2021 | FC Colburn vs Burslem Builders | 2 - 0 | | Recap |
| 2021-08-21 17:00:19August 21, 2021 | Blundell Ballers vs Greenfield Central | 3 - 1 | | Recap |
| 2021-08-21 17:00:31August 21, 2021 | Alton Athletic vs Cambricks United | 4 - 0 | | Recap |
| 2021-08-21 17:00:47August 21, 2021 | Phoenix Shakers vs Whaddon Robins | 0 - 2 | | Recap |
| 2021-08-21 17:00:47August 21, 2021 | Halifax City vs Brunton Wyverns | 0 - 1 | | Recap |
| 2021-08-28 15:00:03August 28, 2021 | Yellowfield Stags vs Prudhoe United | 1 - 4 | | Recap |
| 2021-08-28 15:00:03August 28, 2021 | FC Colburn vs Broadfield Borough | 0 - 0 | | Recap |
| 2021-08-28 15:00:09August 28, 2021 | Holker Street vs Burslem Builders | 0 - 0 | | Recap |
| 2021-08-28 15:00:35August 28, 2021 | Burnden Wanderers vs Blocks County | 0 - 1 | | Recap |
| 2021-08-28 15:00:41August 28, 2021 | Cambricks United vs MonoTown FC | 1 - 2 | | Recap |
| 2021-08-28 15:00:46August 28, 2021 | Somerton Academical vs Amber Exiles | 1 - 4 | | Recap |
| 2021-08-28 15:00:53August 28, 2021 | Bay City Shrimps vs Birkenhead Rovers | 4 - 0 | | Recap |
| 2021-08-28 15:00:55August 28, 2021 | Southsea United vs Alexandra Cruisers | 0 - 2 | | Recap |
| 2021-08-28 18:00:23August 28, 2021 | Alton Athletic vs Brunton Wyverns | 4 - 0 | | Recap |
| 2021-08-28 18:00:34August 28, 2021 | Phoenix Shakers vs Greenfield Central | 3 - 0 | | Recap |
| 2021-08-28 18:00:41August 28, 2021 | Saddle Town Swifts vs Whaddon Robins | 1 - 1 | | Recap |
| 2021-08-28 18:00:45August 28, 2021 | Blundell Ballers vs Halifax City | 4 - 0 | | Recap |
| 2021-09-04 15:00:50September 4, 2021 | MonoTown FC vs Halifax City | 15:00:503:00 pm | MonoTown City StadiumMonoTown | Recap |
